About Hypha Discovery
Hypha Discovery is a UK-based CRO supporting pharmaceutical and agrochemical companies worldwide through the production of metabolites and new derivatives of drugs and agrochemicals in discovery and development. Hypha is an expert in the scalable synthesis and purification of drug metabolites and oxidised derivatives of lead compounds, and possesses a wealth of experience in the production, purification and structure elucidation of natural products.
Follow us on LinkedIn.One-Stop Metabolite Shop
Hypha recently launched the “One Stop Metabolite Shop” which offers clients the opportunity to access phase I and phase II metabolites, from microgram scale for MetID through to multi mg / gram scale for further evaluation and provision of bioanalytical standards.
We offer a comprehensive suite of techniques for provision of even the most difficult-to-synthesise metabolite, comprising chemical synthesis, recombinant enzymes such as PolyCYPs, microbial biotransformation, mammalian liver fractions as well as the purification of metabolites from biological matrices. Hypha scientists are also experts in structural elucidation via NMR spectroscopy.
Read our latest scientific poster illustrating application of the one-stop metabolite shop in meeting metabolite requirements for various client projects using different techniques.
PolyCYPs+ biocatalysis kits
PolyCYPs+ screening kits comprise 18 PolyCYP® isoforms together with the addition of other phase 1 enzymes. Human aldehyde oxidase (AOX1) and the main human hepatic flavin-containing monooxygenase (FMO3) are included in the kit, with other human FMO isoforms available at Hypha. Inclusion of multiple PolyCYPs isoforms broadens coverage of reactions, and which have been proven to metabolise some low turnover drugs and produce multi-step oxidised metabolites.
Download a brochure on PolyCYPs+
Click here to find out more about PolyCYPs+ kits
NEWS & EVENTS
Find out the latest news from Hypha here.
Webinars
We run webinars throughout the year, see our webinar events page to find out about upcoming and past webinars. Coming up on the 12th and 13th November 2020 are webinars on “Hydroxylating drugs: why bother?”
18th – 19th February 2020, Manchester, UK Dr Julia Shanu-Wilson gave a talk on “Integration of biocatalysis into medicinal chemistry programs for late-stage oxidation using PolyCYPs enzymes” Contact us if you would be interested in learning more about this. August 16 – 20 2020, San Francisco, USA Hypha’s CEO Dr Liam Evans will be attending this virtual meeting and giving a talk on “One-stop metabolite shop: Employing multiple tools for accessing metabolites of drugs and agrochemicals” in the session on “Technologies and Predictive Tools for Metabolite Generation, Identification and Assessment.” 25-28th August 2020, San Diego, US Liam will be attending this virtual meeting which focuses on tools and strategies for predicting, optimizing, and Improving PKPD and safety. We’ll be presenting a virtual poster (P16) on interesting case studies involving Hypha’s One Stop Metabolite Shop. October 12th – 16th 2020 Hypha’s Head of Collaborations, Frank Scheffler, is giving a talk at this meeting organised by The Boston Society on “How to Secure Access to Difficult-to-Synthesize Metabolites for MIST Compliance: A One-Step Multiple Tool Approach.“ 26th November 2020 We are sponsoring this fantastic one day online meeting. The scientific program comprises presentations showcasing medicinal chemistry case studies from tools to candidates, across a range of modalities, therapeutic areas and target classes, as well as covering more general topics from the forefront of drug discovery of relevance to medicinal chemists. 4th-5th May 2021, Liverpool, UK. Hypha will be exhibiting at this meeting where Julia will also be giving a talk. 27th – 30th June 2021, New York City, USA Julia and Liam are looking forward to attending this meeting in NYC in 2021.Come and meet us at the following events
SCI/RSC Applied Late-stage Functionalisation: Where Chemistry meets Biology
260th ACS National Meeting & Exposition
Drug Discovery Chemistry: Lead Optimization for Drug Metabolism & Safety
Applied Pharmaceutical Analysis 2020
Hatfield MedChem 2020
5th RSC / DMDG / DMG
New Perspectives in DMPK
ACS Medi 37th National Medicinal Chemistry Symposium
Hypha’s paper in ACS Medicinal Chemistry Letters on “Biotransformation: Impact and Application of Metabolism in Drug Discovery” was published in August 2020. This article discusses the impact and application of biotransformation of drugs by mammalian systems, microorganisms, and recombinant enzymes, covering active and reactive metabolites, the impact of the gut microbiome on metabolism, and how insights gained from biotransformation studies can influence drug design from the combined perspectives of a CRO specializing in a range of biotransformation techniques and pharma biotransformation scientists. Hypha contributed a chapter on “Metabolite Generation and Metabolite Characterization by NMR” in the book published in July 2020 on “Identification and Quantification of Drugs, Metabolites, Drug Metabolizing Enzymes, and Transporters”, edited by Shuguang Ma and Swapan Chowdhury. Download a copy of our brochure on drug metabolites and oxidised derivatives Our brochure on drug metabolites and C-H activated derivatives features interesting case studies and client projects completed using microbial biotransformation. Topics include: Let us know if you would like a copy of a paper published in Xenobiotica entitled Microbial Biotransformation – An Important Tool for the Study of Drug Metabolism, which describes some interesting projects Hypha have undertaken for Merck and Janssen with several difficult-to-synthesise hydroxylated and glucuronidated metabolites. Read the abstract at http://bit.ly/2xb6H9d In a recent newsletter we described a case study illustrating the use of microbial biotransformation to produce scalable quantities of glucuronides, focusing on the synthesis of acyl glucuronides of carboxylic acid-containing drugs. Access a pdf of the newsletter on glucuronides of carboxylic acid-containing drugs Hypha’s PolyCYPs enzymes have been routinely used by pharma and agchem companies for producing metabolites. One application involves direct comparison of radiometric profiles of PolyCYPs and regulatory study samples. In this study by Charles River, a radiolabelled agrochemical compound was incubated with PolyCYPs and the metabolites matched with those detected in goat faeces, as part of an mass balance and disposition study, thereby providing an immediate route to access and scale-up metabolites. Click here for a link to an illustration of the results of this study.More information
Hypha’s latest metabolite project update
