About Hypha Discovery
Hypha Discovery is a UK-based CRO supporting pharmaceutical and agrochemical companies worldwide through the production of metabolites and new derivatives of drugs and agrochemicals in discovery and development. Hypha is an expert in the scalable synthesis, purification and identification of drug metabolites and oxidised derivatives of lead compounds, and possesses a wealth of experience in the production, purification and structure elucidation of natural products.
Follow us on LinkedIn.One-Stop Metabolite Shop
Hypha recently launched the “One Stop Metabolite Shop” which offers clients the opportunity to access phase I and phase II metabolites, from microgram scale for MetID through to multi mg / gram scale for further evaluation and provision of bioanalytical standards.
We offer a comprehensive suite of techniques for provision of even the most difficult-to-synthesise metabolite, comprising chemical synthesis, recombinant enzymes such as PolyCYPs, microbial biotransformation, mammalian liver fractions as well as the purification of metabolites from biological matrices. Hypha scientists are also experts in structural elucidation via NMR spectroscopy.
Download a pdf of our brochure on Hypha’s One-Stop Metabolite Shop
Read our latest scientific poster illustrating application of the one-stop metabolite shop in meeting metabolite requirements for various client projects using different techniques.
PolyCYPs+ biocatalysis kits
PolyCYPs+ screening kits comprise 18 PolyCYP® isoforms together with the addition of other phase 1 enzymes. Human aldehyde oxidase (AOX1) and the main human hepatic flavin-containing monooxygenase (FMO3) are included in the kit, with other human FMO isoforms available at Hypha. Inclusion of multiple PolyCYPs isoforms broadens coverage of reactions, and which have been proven to metabolise some low turnover drugs and produce multi-step oxidised metabolites.
Download a brochure on PolyCYPs+
Click here to find out more about PolyCYPs+ kits
Case Study using PolyCYPs
Hypha’s PolyCYPs enzymes have been routinely used by pharma and agchem companies for producing metabolites. One application involves direct comparison of radiometric profiles of PolyCYPs and regulatory study samples. In this study by Charles River, a radiolabelled agrochemical compound was incubated with PolyCYPs and the metabolites matched with those detected in goat faeces, as part of an mass balance and disposition study, thereby providing an immediate route to access and scale-up metabolites. Click here for a link to an illustration of the results of this study.
NEWS & EVENTS
Find out the latest news from Hypha here.
Webinars
We run webinars throughout the year, see our webinar events page to find out about upcoming and past webinars. Contact us if you want to access recordings on “Hydroxylating drugs: why bother?”
19th – 21st January 2021, online Julia will be attending this online meeting held in partnership with the Swedish Academy of Pharmaceutical Sciences, at which Hypha will be presenting an eposter on the application of multiple metabolite synthesis methods for accessing drug metabolites. 27th January 2020, online “Everything you wanted to discuss about biotransformation and (not) afraid to do so” 9 – 10th March 2021, online Hypha will be presenting at the free online workshop on Metabolite Identification: Strategies, Techniques and Case Studies, organised by PBBS San Francisco on 9th-10th March 2021.
29th April 2021, online We will be presenting as an Exhibitor at this online meeting.
Dates to be advised, online Hypha will be exhibiting at this meeting where Julia will also be giving a talk. The event programme will incorporate sessions themed around the following key topics: 27th – 30th June 2021, New York City, USA Julia and Liam are looking forward to attending this meeting in NYC in 2021.Come and meet us at the following events in 2021
DMDG 2021 Joint Meeting
DMDG 1st European Biotransformation Workshop
Metabolite Identification: Strategies, Techniques and Case Studies Workshop
“Hatfield Medchem Meeting”
32nd Symposium on Medicinal Chemistry in Eastern England
5th RSC / DMDG / DMG
New Perspectives in DMPK
– DMPK and Drug Discovery
– DMPK and Drug Safety
– Analytical and Computational Advances in DMPKACS Medi 37th National Medicinal Chemistry Symposium
Hypha’s paper in ACS Medicinal Chemistry Letters on “Biotransformation: Impact and Application of Metabolism in Drug Discovery” was published in August 2020. This article discusses the impact and application of biotransformation of drugs by mammalian systems, microorganisms, and recombinant enzymes, covering active and reactive metabolites, the impact of the gut microbiome on metabolism, and how insights gained from biotransformation studies can influence drug design from the combined perspectives of a CRO specializing in a range of biotransformation techniques and pharma biotransformation scientists. Hypha contributed a chapter on “Metabolite Generation and Metabolite Characterization by NMR” in the book published in July 2020 on “Identification and Quantification of Drugs, Metabolites, Drug Metabolizing Enzymes, and Transporters”, edited by Shuguang Ma and Swapan Chowdhury. Download a copy of our brochure on drug metabolites and oxidised derivatives Our brochure on drug metabolites and C-H activated derivatives features interesting case studies and client projects completed using microbial biotransformation. Topics include: Let us know if you would like a copy of a paper published in Xenobiotica entitled Microbial Biotransformation – An Important Tool for the Study of Drug Metabolism, which describes some interesting projects Hypha have undertaken for Merck and Janssen with several difficult-to-synthesise hydroxylated and glucuronidated metabolites. Read the abstract at http://bit.ly/2xb6H9d Two of our recent newsletters feature the use of our one-stop metabolite shop to synthesise glucuronide metabolites. In the first one, we describe a case study illustrating the use of microbial biotransformation to produce scalable quantities of glucuronides, focusing on the synthesis of acyl glucuronides of carboxylic acid-containing drugs. Complimenting this is our latest newsletter highlighting the rise in the need to access N-glucuronides and how to synthesise these metabolites. Access a pdf of the newsletter on glucuronides of carboxylic acid-containing drugs Access a pdf of the newsletter on “N-glucuronide metabolites, on the rise?” More information
