Accessing metabolites and derivatives of the agrochemical napropamide

Check out a new white paper describing the joint efforts of Hypha and Galchimia, who together successfully synthesised metabolites of the herbicide napropamide using a complementary approach of chemical synthesis and microbial biotransformation. The joint project was successful in providing a comprehensive family of compounds relevant for the risk assessment of napropamide.

Napropamide is a herbicide approved for use in the EU that is currently undergoing the process for re-registration with the European Food Safety Authority (EFSA). This process typically requires access to metabolites of agrochemicals of interest, which Hypha is able to provide through their One-Stop Metabolite Shop.

In this project, two glucuronidated metabolites, which would have been too complex to prepare by traditional synthetic methods, were made by microbial biotransformation at Hypha. Tens of milligrams of the metabolites were purified by preparative HPLC to >95% purity and their structures confirmed by NMR spectroscopy.