Purification of metabolites from biological matrices
Structure elucidation of metabolites
Hypha are also experts in the structure elucidation of metabolites using NMR spectroscopy. We use cryoporobe technology to gather NMR data on only small amounts of material, and are familiar in interpreting spectra obtained from a wide variety of techniques.
One purification project undertaken at Hypha resulted in tens of milligrams of the R- and S- O– glucuronides of carisbamate, a neuromodulator developed by SK Life Science, which were purified to >95% purity from 150 ml of rabbit urine using a three-step purification method. Confirmation of the structures was obtained by NMR spectroscopy, enabling the use of the purified materials as analytical standards for bioanalysis.
“Hypha Discovery came highly recommended and we subsequently contracted with Hypha to extract and purify metabolites out of rabbit urine. As they were so successful with that extraction, we again requested help for a second program to extract metabolites from human urine and/or synthesize/biosynthesize 3 metabolites. As expected, Hypha has been successful preparing these metabolite reference standards along with structural elucidation and certificates of analysis. In addition, synthesis of one of the metabolites had been attempted at 2 other labs without success; however, Hypha was able to synthesize this difficult metabolite which allowed us to do further evaluations on the metabolite.
Hypha has exceeded our expectations and is now a ‘go to’ lab for biosynthesis/synthesis/purification. Hypha’s team was a pleasure to work with and our complicated projects were handled with expertise and professionalism. Their excellent scientific communication and project data were extremely comprehensive and we received updates throughout the process. We will definitely be Hypha Discovery clients for life”