PolyCYPs® Enzymes

Hypha’s PolyCYPs® enzymes have been developed to provide an easy-to-use in-vitro catalytic system for the rapid oxidation of organic molecules to generate CYP derived metabolites and oxidised derivatives of drugs and agrochemicals.

PolyCYPs® are formatted into screening kits which allow the convenient and cost effective in-house testing of compounds for scalable synthesis of CYP-derived metabolites and hydroxylated derivatives.

PolyCYPs® enzymes are mined from Hypha’s talented microbial biotransformation platform and capable of oxidising a wide range of substrate compounds, especially arylic/benzylic -CH, -CH2, -CH3, -tert-butyl, and iso-propyl moieties, as well as aromatic systems for phenols and epoxides. De-alkylation of N- and O-alkyl moieties is also observed.

Reactions are scalable either by resupply of the enzymes for production in-house, or for larger scale, production / purification at Hypha.

Enzymes in Hypha’s oxidation kit have been rigorously tested for biocatalytic ability against a diverse panel of substrates. So far 84% of substrates tested have been biotransformed to at least one metabolite.

Download a poster presented at the ISSX meeting in Portland US on accessing mammalian metabolites and hydroxylated derivatives of drugs using PolyCYPs enzymes.

Download a pdf of the PolyCYPs brochure.

For more information or to order a kit, email enquiries@hyphadiscovery.co.uk

% drug substrates converted to date = 84%

Key Features

  • Quick access to human and other mammalian metabolites, including active metabolites
  • Discover new hydroxylated derivatives of a wide variety of drug compounds
  • Explore polar chemical space as part of SAR studies
  • Removes the need for speculative synthesis
  • Comes with all reagents needed, just add water
  • Use in-house or outsource to Hypha
  • Scalable

PolyCYPs® metabolite screening kits

View

PolyCYPs® scale-up kits & options

View

PolyCYPs® diversification kits

View

“I see different types of biotransformation, for example, N-dealkylation, aryl hydroxylation, alkyl hydroxylation and N-dearylation.”
US large pharma
“The protocol is very easy to understand and to follow. I appreciated the positive control for the kit for which there was a very high conversion.”
European large pharma
“The system is about as easy as it can be.”
US mid pharma

For more information or to order a kit, email enquiries@hyphadiscovery.co.uk

Back to Top