PolyCYPs® diversification kits
Hypha’s PolyCYPs® diversification kit is a fast and easy way to hydroxylate aliphatic or aromatic moieties at multiple sites in parallel, including metabolically susceptible positions. Both CYP-derived human metabolites and other oxidised derivatives can then be accessed simultaneously for structure elucidation and biological testing.
PolyCYPs® enzymes in the kit have been mined from some of the talented actinomycete bacteria in Hypha’s biotransformation panel, providing a wide diversity of CYPs that hydroxylate a variety of drug compounds.
In this case study, five active monohydroxylated derivatives of a client’s drug lead were produced using enzymes in the diversification kit, with a 56% conversion of the parent drug by the best PolyCYP, enabling access to polar chemical space in parallel as part of SAR studies. Access a pdf of the poster on “Late Stage Functionalization of Drug Candidates using PolyCYPS® Enzymes” here.
For more information or to order a kit, email email@example.com
PolyCYPs diversification kit showing one of the isoforms included