PolyCYPs® and PolyCYPs®+ Kit Instructions
Download PolyCYPs®+ Screening Kit Instructions – part number HDPC-001.2 containing 20 enzymes (18 PolyCYPs, AOX & FMO3) (Nov 19)
Instructions for HDPC-001.2 will be available online soon
Download PolyCYPs Diversification Kit Instructions – part number HDPC-002 (Feb 19)
Incubation conditions
Incubation conditions are important when conducting PolyCYPs reactions to ensure the best % conversions. Examples of suitable incubation conditions are given on page 3 of the instructions. We use a standard 150rpm on a shaker with a 5cm orbit for our PolyCYPs incubations. Other shaker types may have a different orbit which will impact oxygen transfer. Use this handy calculator for determining the best rpm to use for your shaker. You will need to know the orbit of your shaker in cm in order to use the calculator. The calculator works for most types of shakers but please contact us for more advice if your shaker has a very low orbit.
Example:
The MaxQ Mini 4000 benchtop orbital shaker has a throw of 1.9cm. To achieve the same oxygen transfer of an orbital shaker with a throw of 5cm at 150rpm, a speed of 243 rpm is required.
Note that the Eppendorf ThermoMixer has an orbit of only 3mm. We recommend a maximum of 400 rpm if you are using this shaker type, in order to prevent splashing during the incubations.
We have put together a pdf giving some recommendations and tips to help get the most out of the PolyCYPs kits. Read these here.
For help in determining the best conditions for your incubations, email us at support@hyphadiscovery.co.uk
Extract processing
We have put together a suggested procedure for processing PolyCYPs scale-up reactions, along with some handy tips.
Download pdf on processing PolyCYPs extracts for purification of metabolites / derivatives.
For help in determining the best extract procedure for your application, email us at support@hyphadiscovery.co.uk
