PolyCYPs® Kit Instructions
Incubation conditions
Incubtation conditions are important when conducting PolyCYPs reactions. We use a standard 150rpm on a shaker with a 5cm orbit for our PolyCYPs incubations. Other shaker types may have a different orbit which will impact oxygen transfer. Use this handy calculator for determining the best rpm to use for your shaker. You will need to know the orbit of your shaker in cm in order to use the calculator. More information on suitable incubation conditions is given on page 3 of the instructions. For more help in determining the best conditions for your incubations, email us at enquiries@hyphadiscovery.co.uk
Example:
The MaxQ Mini 4000 benchtop orbital shaker has a throw of 1.9cm. To achieve the same oxygen transfer of an orbital shaker with a throw of 5cm at 150rpm, a speed of 243 rpm is required.
Note that the Eppendorf ThermoMixer has an orbit of only 3mm. We recommend a maximum of 400 rpm if you are using this shaker type in order to prevent splashing during the incubations.
For any questions not addressed in the instructions, please email us at enquiries@hyphadiscovery.co.uk
