PolyCYPs® kits for synthesis of CYP derived metabolites

Hypha’s PolyCYPs® screening kit is an easy-to-use in-vitro biocatalytic system for the rapid synthesis of CYP derived human and other mammalian metabolites. Enzymes in the kit typically oxidise -CH, -CH2, -CH3, –tert-butyl, and iso-propyl moieties when attached to benzene or heterocyclic aromatic residues, as well as dealkylating N- and O-alkyl moieties.

Everything is included in the kit that you will need to perform the reaction in your lab, just add water!

The kit consists of enzyme preparations containing recombinant cytochrome P450s together with respective ferredoxin and ferredoxin reductase redox partners, a cofactor recycling system containing glucose-6-phosphate (G6P), nicotinamide adenine dinucleotide phosphate (NADP+), and glucose-6-phosphate-dehydrogenase (G6PDH).

In addition to the enzyme preparations, a cyclodextrin-based formulation reagent to aid the solubilisation of test compounds of poor aqueous solubility is included together with a positive control substrate.

For more information or to order a kit, email enquiries@hyphadiscovery.co.uk

Download a pdf of the PolyCYPs brochure.

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