PolyCYPs®+ Metabolite Screening Kits

PolyCYPs®+ kits for synthesis of CYP and other phase I derived metabolites

Hypha’s PolyCYPs®+ screening kit is an easy-to-use in-vitro biocatalytic system for the rapid synthesis of oxidised human and other mammalian metabolites of drugs and agrochemicals. Enzymes in the kit typically oxidise -CH, -CH2, -CH3, –tert-butyl, and iso-propyl moieties when attached to benzene or heterocyclic aromatic residues, as well as dealkylating N- and O-alkyl moieties.

PolyCYPs®+ kit contains more than double the number of CYPs contained in the original kit, and is broadened by the addition of other phase I enzymes. Human aldehyde oxidase (AOX1) and the main human hepatic flavin-containing monooxygenase (FMO3) are now included in the kit, with other human FMO isoforms available soon. Inclusion of new PolyCYPs isoforms expands coverage of reactions, and have been proven to metabolise some low turnover drugs and produce multi-step oxidised metabolites.

Read more about PolyCYPs+ here.

PolyCYPs+ kit content

The kit consists of enzyme preparations of 18 PolyCYPs enzymes, AOX1 and FMO3. Also included are ferredoxin and ferredoxin reductase redox partners, a cofactor recycling system containing glucose-6-phosphate (G6P), nicotinamide adenine dinucleotide phosphate (NADP+), and glucose-6-phosphate-dehydrogenase (G6PDH), for use with PolyCYPs and FMO3 enzymes.

In addition to the enzyme preparations, a cyclodextrin-based formulation reagent to aid the solubilisation of test compounds of poor aqueous solubility is included together with a positive control substrate and enzyme.

All reagents are provided as lyophilised powders in sealed vials together with a 24-well reaction plate and air-permeable seal.

Everything is included in the kit that you will need to perform the reaction in your lab, just add water!

For more information or to order a kit, email enquiries@hyphadiscovery.co.uk

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