PolyCYPs® scale up options
- Provision of multiple quantities of any PolyCYP enzyme lyophilised in vials in a kit format for use in your own labs.
- For large quantities, we offer reactions undertaken at Hypha using cell paste from the recombinant E.coli clone containing the CYP of interest, or via fermentation with a Streptomycete clone. Contact us to find out more about this option at enquiries@hyphadiscovery.co.uk.
Multiple 10ml scale-up vials, suitable for converting 1mg of parent per vial, can be ordered off-the-shelf for any isoform for in-house scale-up. Co-factors are included. Each 10ml scale-up vial kit is supplied with a 24-well block suitable for performing 4 x 2.5 ml reactions. Other incubation formats can also be used, as recommended in the instructions.
Hypha also offer a service option should you prefer us to undertake scale-up reactions from vials, or to process and purify metabolites / derivatives from reaction extracts. We also have expertise in structure elucidation of products using NMR spectroscopy.
Case Studies
- In this client case study, milligram amounts of specific oxidised products of two compounds were produced using two different PolyCYPs enzymes. Both screening and scale-up reactions were conducted by Hypha for the client and the extract provided to the client for purification. Click here to view client case study.
- In the experiment in the link below, PolyCYP 152 was used to illustrate scale-up production of the two major human hydroxylated metabolites of diclofenac. The reactions were performed in 2.5ml volumes using three different batches of the PolyCYP enzyme with excellent reproducibility. A higher % conversion was observed in the 2.5ml reactions vs the 0.5ml screening volume reaction. View the diclofenac scale-up reaction example.
For more information on the options available, email enquiries@hyphadiscovery.co.uk
Extract processing
We have put together a suggested procedure for processing PolyCYPs scale-up reactions, along with some handy tips.
Access pdf on processing PolyCYPs extracts for purification of metabolites / derivatives here.
For help in determining the best extract procedure for your application, email us at support@hyphadiscovery.co.uk
PolyCYPs scale-up vial