PolyCYPs® scale up options
- Provision of multiple quantities of any PolyCYP enzyme lyophilised in vials in a kit format for use in your own labs.
- For large quantities, we offer reactions undertaken at Hypha using cell paste from the recombinant E.coli clone containing the CYP of interest, or via fermentation with a Streptomycete clone. Contact us to find out more about this option.
Multiple 10ml scale-up vials, suitable for converting 1mg of parent per vial, can be ordered off-the-shelf for any isoform for in-house scale-up. In the case study in the link below, PolyCYP152 was used to scale-up production of the two major human hydroxylated metabolites of diclofenac. The reactions were performed in a 2.5ml volumes. Each 10ml scale-up vial kit is supplied with a 24-well block suitable for performing 4 x 2.5 ml reactions. Other incubation formats can also be used, as recommended in the instructions.
View scale-up vial case study here.
For more information on the options available, email enquiries@hyphadiscovery.co.uk
Extract processing
We have put together a suggested procedure for processing PolyCYPs scale-up reactions, along with some handy tips.
Access pdf on processing PolyCYPs extracts for purification of metabolites / derivatives here.
Hypha also offer a service option should you prefer us to undertake scale-up reactions from vials, or to process and purify metabolites / derivatives from reaction extracts. We also have expertise in structure elucidation of products using NMR spectroscopy.
For help in determining the best extract procedure for your application, email us at support@hyphadiscovery.co.uk
PolyCYPs scale-up vial