PolyCYPs® scale up options
PolyCYPs® reactions are fully scalable from mg to gram quantities through a number of routes.
- Provision of multiple quantities of any PolyCYP enzyme lyophilised in vials in a kit format for use in your own labs.
- For large quantities, we offer reactions undertaken at Hypha using cell paste from the recombinant E.coli clone containing the CYP of interest, or via fermentation with a Streptomycete clone. Contact us to find out more about this option.
In-house scale-up option
Mulitple 10ml scale-up vials, suitable for converting 1mg of parent per vial, can be ordered off-the-shelf for any isoform. In the case study in the link below, PolyCYP152 was used to scale-up production of the two major human hydroxylated metabolites of diclofenac. The reactions were performed in a 2.5ml volumes. Each scale-up vial kit is supplied with a 24-well block suitable for performing 4 x 2.5 ml reactions. Other incubation formats can also be used, as recommended in the instructions.
View scale-up vial case study here.
For more information on the options available, email enquiries@hyphadiscovery.co.uk
PolyCYPs scale-up vial